卜子梅
Professor and Laboratory Director
Department of Chemistry
Marshak Science Building 1336
City College of New York
160 Convent Avenue
New York, NY 10031
zbu at ccny dot cuny dot edu
Looking for a
WELL-COMPENSATED
job?
My main page is
HERE!
Nanoscale
protein dynamics:
A new
frontier
for neutron spin echo spectroscopy
Neutron spin echo
spectroscopy
(NSE)
is the
only experimental technique
that can determine protein motion on nano length and time scales.
Such motions have long been hypothesized to be the most significant modes for protein function.
We have developed a theoretical framework that allows one to interpret the NSE data.
Our theoretical framework uses simple concepts from
nonequilibrium statistical mechanics,
and does not require elaborate molecular dynamics simulations, complex fits to rotational motion,
or elastic network models. In combination with our experimental methodology of selective deuterium
labeling, our work clears significant hurdles to the understanding of this very important problem.
Apply
HERE
for jobs
Click
HERE!
to see opportunities
cover
article
A brief introduction to
neutron spin echo spectroscopy
and protein dynamics
HERE
Another recent publication HERE! and HERE! and HERE!
Dynamic regimes and correlated structural dynamics in native and denatured alpha-lactalbumin:
A quasielastic neutron scattering study.
DOI: 10.1006/jmbi.2001.5006
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Another recent publication HERE! and HERE!
Ezrin induces long-range interdomain allostery in the scaffolding protein NHERF1
Jianquan Li, David J.E. Callaway,and Zimei Bu. J Mol Biol. Sep 11, 2009; 392(1): 166?180.
DOI: 10.1016/j.jmb.2009.07.005
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Another recent publication and HERE! and HERE!
Structure-specific DNA induced conformational changes in Taq polymerase revealed by small angle neutron scattering.
DOI: 10.1074/jbc.M404565200
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Another recent publication HERE! and HERE! and HERE!
A conformational switch in the scaffolding protein NHERF1 controls autoinhibition and complex formation.
DOI: 10.1074/jbc.M109.074005
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Another recent publication HERE! and HERE!
Ezrin controls the macromolecular complexes formed between
an adapter protein Na+/H+ exchanger regulatory factor (NHERF1) and the
cystic fibrosis transmembrane conductance regulator (CFTR).
Li J, Dai Z, Jana D, Callaway DJ, Bu Z. J Biol Chem. 2005 Nov 11;280(45):37634-43.
DOI: 10.1074/jbc.M502305200
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Another recent publication HERE! and HERE! and HERE!
Nanoscale protein domain motion and long-range allostery in signaling proteins:
A view from neutron spin echo spectroscopy
Biophysical Reviews ISSN 1867-2450
DOI: 10.1007/s12551-015-0162-x
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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE!
Essential strategies for revealing nanoscale protein dynamics by neutron spin echo spectroscopy
Callaway DJE, Bu Z. Methods in Enzymology.
Isotope Labeling of Biomolecules - Applications. Volume 566, 2016, Pages 253 -270.
ISBN: 978-0-12-803049-3
DOI: 10.1016/bs.mie.2015.05.011
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Another recent publication HERE! and HERE! and HERE! and HERE!
Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering.
Jayasundar JJ, Ju JH, He L, Liu D, Meilleur F, Zhao J, Callaway DJ, Bu Z. J Biol Chem. 2012 Oct 26;287(44):37119-33.
DOI: 10.1074/jbc.M112.380972
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Another recent publication HERE! and HERE!
Protein kinase C phosphorylation disrupts Na+/H+ exchanger regulatory factor 1 autoinhibition and promotes cystic fibrosis transmembrane conductance regulator macromolecular assembly.
Li J, Poulikakos PI, Dai Z, Testa JR, Callaway DJ, Bu Z. J Biol Chem. 2007 Sep 14;282(37):27086-99
DOI: 10.1074/jbc.M702019200
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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE! and HERE!
Visualizing the nanoscale: Protein internal dynamics and neutron spin echo spectroscopy
cover
article
Callaway DJE, Bu Z. Current Opinion in Structural Biology 2017,42:1-5.
DOI: 10.1016/j.sbi.2016.10.001
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Another recent publication HERE! and HERE! and HERE!
Phosphatidylinositol 4,5-bisphosphate clusters the cell adhesion molecule CD44 and assembles a specific CD44-Ezrin heterocomplex, as revealed by small angle neutron scattering
Xiaodong Chen, Jahan Ali Khajeh, Jeong Ho Ju, Yogesh K. Gupta, Christopher B. Stanley, Changwoo Do, William T. Heller, Aneel K. Aggarwal,
David J. E. Callaway and Zimei Bu
J Biol Chem. 2015 Mar 6;290(10):6639-52
DOI: 10.1074/jbc.M114.589523
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Another recent publication HERE! and HERE! and HERE!
Assembling amyloid fibrils from designed structures
containing a significant Alzheimer beta-peptide fragment.
L.O. Tjernberg,
A. Tjernberg,
N. Bark,
Y. Shi,
B. P. Ruzsicska,
Z. Bu,
J. Thyberg,
D.J.E. Callaway.
Biochem. J. (2002) 366, 343-351.
Another recent publication HERE! and HERE!
Shi Y, Stouten PF,
Pillalamarri N, Barile L,
Rosal RV, Teichberg S, Bu Z,
D.J.E. Callaway.
Quantitative determination
of the topological propensities
of amyloidogenic peptides.
Biophys Chem. 120 (2006), 55-61.
Another recent publication HERE! and HERE! and HERE!
Bu Z, Shi Y,
D.J.E. Callaway, {co-corresponding},
and Tycko R.
Molecular alignments within
beta-sheets in Abeta(14-23) fibrils:
solid-state NMR experiments and theoretical predictions.
Biophys J. 2007 92: p. 594-602.