David J. E. Callaway, PhD
Professor and Laboratory Director
大卫★凯乐威

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Biological NanoPhysics
"The Future of Medicine "

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EXCEPTIONAL compensation for EXCEPTIONAL individuals

"There's plenty of room at the bottom: An invitation to enter a new field of PHYSICS."
-- Richard Feynman, 1959

  

NOW we can watch protein nanomachines MOVE
with Neutron Spin Echo spectroscopy!

Protein nanomachines are far more complex than any molecular machines yet constructed.
Their skillful utilization likely represents the future of medicine.


★Easy introduction to NSE HERE★

Nanoscale protein dynamics: A new frontier for
Neutron Spin Echo spectroscopy


Neutron spin echo spectroscopy (NSE) is the only experimental technique that can determine protein motion on nano length and time scales. Such motions have long been hypothesized to be the most significant modes for protein function. We have developed a theoretical framework that allows one to interpret the NSE data. Our theoretical framework uses simple concepts from nonequilibrium statistical mechanics, and does not require elaborate molecular dynamics simulations, complex fits to rotational motion, or elastic network models. In combination with our experimental methodology of selective deuterium labeling, our work clears significant hurdles to the understanding of this very important problem.

Read all about it HERE!

Interdisciplinary nanoscale biophysical approaches to the cure of disease were once thought revolutionary, but are now rapidly becoming a standard part of the repertoire. We have instituted a new multidisciplinary program of research in this area, headed by Professor David Callaway.

Dr Callaway has worked extensively in the field of Alzheimer's disease, specifically in understanding and controlling amyloid formation. His Alzheimer's program has developed and patented several potential therapeutics for Alzheimer's disease, based upon apomorphine, which are reviewed in a Wikipedia article.

Trained as a theoretical physicist, Dr. Callaway has published research on topics ranging from elementary particles, black holes, semiconductors and superconductors to protein dynamics and structure-based drug design. Presently he works on probing protein domain dynamics. by neutron spin-echo spectroscopy., allowing us to observe protein nanomachines in motion.

In his ★copious★ free time, he sails the world and climbs its mountains.

Climbing in the Alps, Himalaya, Karakoram, assorted greater ranges, personal web page, other adventures, and other stuff.


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A recent publication HERE! and HERE! and HERE! and HERE! and HERE!
Proteins MOVE! Protein dynamics and long-range allostery in cell signaling.

Bu Z, Callaway DJE. Adv Protein Chem Struct Biol. 2011;83:163-221.
PMID: 21570668
DOI: 10.1016/B978-0-12-381262-9.00005-7


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Another recent publication HERE! and HERE! and HERE! and HERE!
Activation of nanoscale allosteric protein domain motion revealed by neutron spin echo spectroscopy.




Farago B, Li J, Cornilescu G, Callaway D.J.E. (co-corresponding), Bu Z.
Biophys J. 2010 Nov 17;99(10):3473-82.
DOI: 10.1016/j.bpj.2010.09.058


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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE!
Nanoscale protein dynamics: A new frontier for neutron spin echo spectroscopy,




Callaway DJ, Farago B, Bu Z.
The European Physical Journal E-Soft Matter & Biological Physics
Eur. Phys. J. E (2013) 36: 76
DOI: 10.1140/epje/i2013-13076-1


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Another recent publication HERE! and HERE! and HERE! and HERE!
Coupled protein domain motion in Taq polymerase revealed by neutron spin-echo spectroscopy.

Bu Z, Biehl R, Monkenbusch M, Richter D, Callaway D.J.E. (co-corresponding)
Proc Natl Acad Sci USA 2005 Dec 6;102(49):17646-17651.
DOI: 10.1073/pnas.0503388102


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Another recent publication HERE! and HERE! and HERE!
Dynamic regimes and correlated structural dynamics in native and denatured alpha-lactalbumin:
A quasielastic neutron scattering study.
Z. Bu, J. Cook, D.J.E. Callaway. J. Mol. Biol. (2001) 312, 865-873.
DOI: 10.1006/jmbi.2001.5006


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Another recent publication HERE! and HERE!
Ezrin induces long-range interdomain allostery in the scaffolding protein NHERF1


Jianquan Li, David J.E. Callaway,and Zimei Bu. J Mol Biol. Sep 11, 2009; 392(1): 166?180.
DOI: 10.1016/j.jmb.2009.07.005


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Another recent publication and HERE! and HERE!
Structure-specific DNA induced conformational changes in Taq polymerase revealed by small angle neutron scattering.
D. L. Ho, W. M. Byrnes, W. P. Ma, Y. Shi, D.J.E. Callaway (co-corresponding), Zimei Bu. J. Biol. Chem., 2004 Sep 10;279(37):39146-54.
DOI: 10.1074/jbc.M404565200


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Another recent publication HERE! and HERE! and HERE!
A conformational switch in the scaffolding protein NHERF1 controls autoinhibition and complex formation.
Bhattacharya S, Dai Z, Li J, Baxter S, D.J.E. Callaway (co-corresponding), Cowburn D, Bu Z. J Biol Chem. 2010 Mar 26;285(13):9981-94.
DOI: 10.1074/jbc.M109.074005


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Another recent publication HERE! and HERE!
Ezrin controls the macromolecular complexes formed between
an adapter protein Na+/H+ exchanger regulatory factor (NHERF1) and the
cystic fibrosis transmembrane conductance regulator (CFTR).

Li J, Dai Z, Jana D, Callaway DJ, Bu Z. J Biol Chem. 2005 Nov 11;280(45):37634-43.
DOI: 10.1074/jbc.M502305200


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Another recent publication HERE! and HERE! and HERE!
Nanoscale protein domain motion and long-range allostery in signaling proteins:
A view from neutron spin echo spectroscopy.

Callaway DJE, Bu Z. Biophys Rev. 2015 Jun;7(2):165-174.
Biophysical Reviews ISSN 1867-2450
DOI: 10.1007/s12551-015-0162-x


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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE!
Essential strategies for revealing nanoscale protein dynamics by neutron spin echo spectroscopy

Callaway DJE, Bu Z. Methods in Enzymology.
Isotope Labeling of Biomolecules - Applications. Volume 566, 2016, Pages 253 -270.
ISBN: 978-0-12-803049-3
DOI: 10.1016/bs.mie.2015.05.011


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Another recent publication HERE! and HERE! and HERE! and HERE!
Open conformation of ezrin bound to phosphatidylinositol 4,5-bisphosphate and to F-actin revealed by neutron scattering.

Jayasundar JJ, Ju JH, He L, Liu D, Meilleur F, Zhao J, Callaway DJ, Bu Z. J Biol Chem. 2012 Oct 26;287(44):37119-33.
DOI: 10.1074/jbc.M112.380972


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Another recent publication HERE! and HERE!
Protein kinase C phosphorylation disrupts Na+/H+ exchanger regulatory factor 1 autoinhibition and promotes cystic fibrosis transmembrane conductance regulator macromolecular assembly.

Li J, Poulikakos PI, Dai Z, Testa JR, Callaway DJ, Bu Z. J Biol Chem. 2007 Sep 14;282(37):27086-99
DOI: 10.1074/jbc.M702019200


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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE! and HERE!
Visualizing the nanoscale: Protein internal dynamics and neutron spin echo spectroscopy
 cover   article 
Callaway DJE, Bu Z. Current Opinion in Structural Biology 2017,42:1-5.
DOI: 10.1016/j.sbi.2016.10.001


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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE!
Controllable activation of nanoscale dynamics in a disordered protein alters binding kinetics.

David J E Callaway (co-corresponding), Tsutomu Matsui, Thomas Weiss, Laura R Stingaciu, Christopher B Stanley, William T Heller, Zimei Bu.
Journal of Molecular Biology 429 (2017) pp. 987-998
DOI: 10.1016/j.jmb.2017.03.003


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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE!
Alpha-catenin structure and nanoscale dynamics in solution and in complex with F-actin

Iain D Nicholl, Tsutomu Matsui, Thomas M Weiss, Christopher B Stanley, William T Heller, Anne Martel, Bela Farago,
David J E Callaway (co-corresponding), Zimei Bu
Biophys. J. 115(4), 642-654, August 21, 2018
DOI: 10.1016/j.bpj.2018.07.005


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Another recent publication HERE! and HERE! and HERE!
Phosphatidylinositol 4,5-bisphosphate clusters the cell adhesion molecule CD44 and assembles a specific CD44-Ezrin heterocomplex, as revealed by small angle neutron scattering

Xiaodong Chen, Jahan Ali Khajeh, Jeong Ho Ju, Yogesh K. Gupta, Christopher B. Stanley, Changwoo Do, William T. Heller, Aneel K. Aggarwal,
David J. E. Callaway and Zimei Bu
J Biol Chem. 2015 Mar 6;290(10):6639-52
DOI: 10.1074/jbc.M114.589523


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Another recent publication HERE! and HERE! and HERE!
Neutron scattering in the biological sciences: progress and prospects

Ashkar, R., Bilheux, H., Bordallo, H., Briber, R., Callaway, D.J.E., et al.
Acta Cryst. (2018). D74, 1129-1168
DOI: 10.1107/S2059798318017503

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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE!
An ensemble of flexible conformations underlies mechanotransduction by the cadherin-catenin adhesion complex

Proc Natl Acad Sci USA 2019
Bush M, Alhanshali BM, Qian S, Stanley C, Heller W, Matsui T, Weiss T, Nicholl ID, Walz T, Callaway DJE (co-corresponding), Bu Z.
DOI: 10.1073/pnas.1911489116

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Another recent publication HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE! and HERE!
Activated nanoscale actin-binding domain motion in the catenin-cadherin complex revealed by neutron spin echo spectroscopy

Proc Natl Acad Sci USA
March 30, 2021 118 (13) e2025012118
Farago B, Nicholl ID, Wang S, Cheng X, Callaway DJE (co-corresponding), Bu Z
DOI: 10.1073/pnas.2025012118

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New horizons: unanticipated ultrafast dynamics in cytoskeletal signaling
and the motorman model


The highly dynamic nature of protein complexes


Callaway DJE (co-corresponding), Nicholl ID, Shi B, Reyes G, Farago B, Bu Nanoscale dynamics of the cadherin-catenin complex bound to vinculin revealed by neutron spin echo spectroscopy Proceedings of the National Academy of Sciences of the United States of America September 19, 2024121 (39) e2408459121 volume = 129 issue = 39 date =2024 pmid =39298480 doi = 10.1073/pnas.2408459121 url = https://doi.org/10.1073/pnas.2408459121

Available HERE! and HERE! and HERE!

Looking for a WELL-COMPENSATED job? Click HERE!

欢迎访问学者客座教授




Alzheimer's program HERE! with my
million dollar baby.



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 ★★★★

Potential therapeutics for Alzheimer's disease

discovered in our laboratory


HERE! and HERE! and HERE! and HERE! and HERE! and HERE!
New class of beta-amyloid aggregation inhibitors: Implications for the mechanism of pathogenesis in Alzheimer's disease.
H. A. Lashuel, D. M. Hartley, D. Balakhaneh, A. Aggarwal, S. Teichberg, D.J.E. Callaway. J. Biol. Chem., (2002) 277,42881-42890.
HERE! and HERE! and HERE! Assembling amyloid fibrils from designed structures containing a significant Alzheimer beta-peptide fragment. L.O. Tjernberg, A. Tjernberg, N. Bark, Y. Shi, B. P. Ruzsicska, Z. Bu, J. Thyberg, D.J.E. Callaway. Biochem. J. (2002) 366, 343-351.
HERE! and HERE! and HERE! and HERE! and HERE! Solvent-induced organization: a physical model of folding myoglobin. Proteins. 1994 Oct;20(2):124-38. D.J.E. Callaway. cond-mat 9406071
HERE! and HERE! Shi Y, Stouten PF, Pillalamarri N, Barile L, Rosal RV, Teichberg S, Bu Z, D.J.E. Callaway, Quantitative determination of the topological propensities of amyloidogenic peptides. Biophys Chem. 120 (2006), 55-61.


HERE! and HERE! and HERE! Bu Z, Shi Y, D.J.E. Callaway, {co-corresponding}, and Tycko R. Molecular alignments within beta-sheets in Abeta(14-23) fibrils: solid-state NMR experiments and theoretical predictions. Biophys J. 2007 92: p. 594-602.
HERE! and HERE! Controlling amyloid beta-peptide fibril formation with protease stable ligands. J. Biol. Chem. 1997 272 (19): 12601-12605; 272 (28): 17894.
HERE! and HERE! A molecular model of Alzheimer amyloid beta-peptide fibril formation. J. Biol. Chem. 1999 274, 12619-12625.
HERE! The amyloid beta peptide (Abeta(1-40)) is thermodynamically soluble at physiological concentrations. Biochemistry. 2003 Sep 9;42(35):10506-13.

High energy, nuclear, statistical, condensed matter and computational physics

HERE! and HERE and HERE and HERE and HERE and HERE Triviality pursuit: Can elementary scalar particles exist? D.J.E. Callaway, Phys. Reports 167 (1988), 241.

HERE! and HERE and HERE and HERE and HERE On the remarkable structure of the superconducting intermediate state. D.J.E. Callaway, Nucl. Phys. B344 [FS] (1990) 627.

HERE! and HERE and HERE Langevin simulations in Minkowski space. D.J.E. Callaway, F. Cooper, J. R. Klauder, H. Rose. Nucl.Phys.B262:19,1985

(PDF format) and HERE and HERE Can Elementary Scalar Particles Exist? D.J.E. Callaway, R. Petronzio. Nuclear Physics B267(1986),253-265

HERE Can Elementary Scalar Particles Exist? 2. Scalar Electrodynamics D.J.E. Callaway, R. Petronzio. Nucl.Phys. B277 (1986) 50 CERN-TH-4369/86, RU86/B/151 DOI: 10.1016/0550-3213(86)90431-1

HERE! and arxiv and HERE and HERE and HERE Surface tension, hydrophobicity, and black holes: The entropic connection. D.J.E. Callaway, Phys. Rev. E53 (1996), 3738.

HERE! and HERE and HERE and HERE and HERE. Random matrices, fractional statistics, and the quantum Hall effect. D.J.E. Callaway, Phys. Rev. B43 (1991) 8641.


HERE! and HERE and HERE and HERE Microcanonical ensemble formulation of lattice gauge theory. D.J.E. Callaway and A. Rahman, Phys. Rev. Lett. 49 (1982), 613.

HERE and HERE and HERE David J. E. Callaway and Aneesur Rahman (1983). Lattice gauge theory in the microcanonical ensemble. Physical Review. D28 (6): 1506 1514. Bibcode:1983PhRvD..28.1506C. doi:10.1103/PhysRevD.28.1506.

HERE! and HERE and HERE and HERE and HERE Non-triviality of gauge theories with elementary scalars and upper bounds on Higgs masses. D.J.E. Callaway, Nucl. Phys. B233 (1984), 189.

HERE! and HERE and HERE and HERE and HERE Is the standard model Higgs mass predictable? D.J.E. Callaway and R. Petronzio, Nuclear Physics B, Volume 292, p. 497-526 (1987).

HERE! and HERE and HERE and HERE and HERE Determination of critical points and flow diagrams by Monte Carlo renormalization group methods. D.J.E. Callaway and R. Petronzio, Phys. Lett. 139B (1984), 189.

HERE! and HERE and HERE Spin structure of the nucleon. D.J.E. Callaway and S.D. Ellis Phys. Rev. D 29 (1984), 567.

HERE! and HERE and HERE and HERE Effective potential of lattice phi^4 theory. D.J.E. Callaway and D.J. Maloof, Phys. Rev. D27 (1983), 406.

HERE! and HERE and HERE and HERE and HERE Abelian Higgs model: A Monte Carlo study. D.J.E. Callaway and L.J. Carson, Phys. Rev. D25 (1982), 531.

HERE! Topics in Lattice Gauge Theory. Lectures for Non-Particle Theorists given to the Materials Science and Technology Division at Argonne National Laboratory. D.J.E. Callaway, Argonne Report ANL-HEP-82-57, November 1982.

HERE! and (PDF format) and HERE and HERE and HERE and HERE Landau, Abrikosov, Hofstadter: Magnetic Flux Penetration in a Lattice Superconductor David J.E. Callaway Annals Phys. 224 (1993) 210-224 DOI: 10.1006/aphy.1993.1045 arXiv:hep-lat/9301013


Protein nanomachines are far more complex than any molecular machines yet constructed.
Their skillful utilization likely represents the future of medicine.

Looking for a WELL-PAID job? Want to get a PhD? Want a summer internship?
Click HERE!


Why neutrons?

See research on ResearchGate, research on Academia●edu, research in high energy, nuclear, condensed matter and computational physics, research in biological nanophysics, research on Google Scholar, research on Scopus, research at CERN library, research at INSPIRE-HEP, profile on LinkedIn, profile on Facebook, profile on Wikipedia, Net Worth, personal web page, and Google site web page.


Climbing in the Alps, Himalaya, Karakoram, assorted greater ranges, personal web page, other adventures, and other stuff.

Biophysical Society poster 2019

Biophysical Society poster 2018

What next?

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